The oxygen microsensors from PyroScience feature a small tip of only 50 - 70 µm which enables fast and precise measurements in small samples with high spatial resolution. Our fiber-optic oxygen sensors feature no oxygen consumption, no stirring sensitivity, an extremely long shelf time, resistance to corrosive environments (e.g. seawater) and are suitable for multiple applications in gas, water and aqueous samples.

Common application areas are:

• Measurements in low/small volumes
• Microprofiling e.g., in sediments, biofilms or 3D cell cultures
• Measurement in plant tissues
• Aquatic Eddy Covariance measurement

Microsensors can be used with our multi-channel PC-operated FireSting®-O2, the multi-analyte meter FireSting®-PRO (also in combination with our optical pH sensors), or stand-alone with our pocket oxygen meter FireSting®-GO2. Furthermore, underwater SUB connectors allow to connect the sensors to our AquaPHOX devices for measurements under water even in the deep sea (down to 4000m).

Applicable Oxygen Sensor Types

Fixed Fiber Sensor (OXF50): The fragile sensor tip protrudes ca. 6mm from a straight-cut needle. The sensor can be applied in liquids and in gases. This sensor type is best suited for application in media with high salt content (e.g. seawater) and can be used for micro profiling of biofilms.

Retractable Needle-Type Sensor (OXR50): The fragile sensor tip is surrounded by a 40 mm long syringe needle. The sensor tip can be extended from the needle for sensor calibration and measurements. Therefore, the sensor tip is protected during handling of the sensor like for example insertion into measuring set-ups. Once fixed in the set-up, e.g. for microprofiling the sensor tip can be extended from the protecting needle (for up to 12mm) allowing  measurements and profiling applications at high spatial and temporal resolution.

Bare Fiber Sensor (OXB50): This sensor is based on the same fiber as the other needle-type microsensors, but it comes without sensor housing. The bare fiber sensors are flexible and enable, e.g. integration into customized housings with complex geometries or implantation into sediments, soils or plant tissue.

Applicable Oxygen Sensor Options and Accessories

• High speed (-HS) (<0.8s response time) and Ultra-high speed (-UHS) (<0.3s response time) for very fast measurements (e.g. aquatic Eddy covariance measurements)
• Optical isolation (-OI) for measurements with high illumination (e.g. photosynthesis measurements)
• SUB connector(-SUB) for measurements under water using an APHOX device
• Microprofiling set-ups (MU1, MM33, MUX2, HS1, LS1) (e.g. profiling of sediments, biofilms or cell cultures)

Related Publications

Fate-mapping post-hypoxic tumor cells reveals a ROS-resistant phenotype that promotes metastasis
Godet et al., 2019, Nature Communications
https://www.nature.com/articles/s41467-019-12412-1

Hyperbaric Oxygen Sensitizes Anoxic Pseudomonas aeruginosa Biofilm to Ciprofloxacin
Kolpen et al., 2017, Antimicrob Agents Chemother.
https://journals.asm.org/doi/full/10.1128/aac.01024-17

Comparison of Engineered Liver 3D Models and the Role of Oxygenation for Patient-Derived Tumor Cells and Immortalized Cell Lines Cocultured with Tumor Stroma in the Detection of Hepatotoxins
Mansouri et al., 2023, Advanced Biology
https://onlinelibrary.wiley.com/doi/full/10.1002/adbi.202300386

A New Technique for Resolving Benthic Solute Fluxes: Evaluation of Conditional Sampling Using Aquatic Relaxed Eddy Accumulation
Calabro-Souza et al., 2023, Earth and Space Science
https://doi.org/10.1029/2023EA003041

Oxygenation of Hypoxic Coastal Baltic Sea Sediments Impacts on Chemistry, Microbial Community Composition, and Metabolism
Broman et al., 2017, Front. Microbiol
https://doi.org/10.3389/fmicb.2017.02453

Development of a rechargeable optical hydrogen peroxide sensor – sensor design and biological application
Koren et al., 2016, Analyst
https://doi.org/10.1039/C6AN00864J

The Reduction of Dissolved Oxygen During Magnesium Corrosion
Silva et al., 2018, Chemistry Open
https://doi.org/10.1002/open.201800076

Suitability of Contact-Free Oxygen Optical Microsensors for Measuring Respiration and Photosynthesis in Green Algae
Zhang et al., 2017, Front. Environ.Sci
https://doi.org/10.3389/fenvs.2017.00091

Composition of Photosynthetic Gas Bubbles From Submerged Macrophytes
Shikhani et al., 2024, Water Resources Research
https://doi.org/10.1029/2022WR034010

Bacterial aerobic respiration is a major consumer of oxygen in sputum from patients with acute lower respiratory tract infection
Jensen et al., 2024 ,APMIS.
https://doi.org/10.1111/apm.13381

Implications of Glacial Melt-Related Processes on the Potential Primary Production of a Microphytobenthic Community in Potter Cove (Antarctica)
Hoffmann et al., 2019, Fron. Mar. Sci.
https://www.frontiersin.org/articles/10.3389/fmars.2019.00655/full

Hyperbaric oxygen treatment increases killing of aggregating Pseudomonas aeruginosa isolates from cystic fibrosis patients
Møller et al., 2019, Journal of Cystic Fibrosis
https://doi.org/10.1016/j.jcf.2019.01.005

Fast cycling of intermittent hypoxia in a physiomimetic 3D environment: A novel tool for the study of the parenchymal effects of sleep apnea
Jurado et al., 2023, Frontiers in Pharmacology
https://doi.org/10.3389/fphar.2022.1081345

Hypoxic Conditions in Crown Galls Induce Plant Anaerobic Responses That Support Tumor Proliferation
Kerpen et al., 2019, Frontiers in Plant Science
https://doi.org/10.3389/fpls.2019.00056

Aquatic Eddy Correlation: Quantifying the Artificial Flux Caused by Stirring-Sensitive O2 Sensors
Holtappels et al., 2015, PLOS ONE
https://doi.org/10.1371/journal.pone.0116564